Purification of industrial ?amylase by reversed micellar extraction
نویسندگان
چکیده
منابع مشابه
Parameters affecting the efficiency of affinity-based reversed micellar extraction and separation (ARMES) in glycoprotein purification.
Affinity-based reversed micellar extraction and separation (ARMES) is an effective method for purifying both low and high molecular weight glycoproteins via liquid-liquid extraction. A range of extraction conditions were examined to gain insight into the mechanism of ARMES. Concanavalin A (Con A) was used as the model affinity ligand to bind soybean peroxidase (SBP) and beta-galactosidase as mo...
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( ) Affinity-based re®ersed micellar extraction and separation ARMES has pro®en effecti®e in separating glycoproteins from nonglycosylated proteins from natural sources. The ability of ARMES to resol®e closely related glycoproteins is of paramount importance if ARMES is to be used in glycoform resolution. It is demonstrated that ARMES ( ) can resol®e the structurally similar soybean peroxidase ...
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Background and purpose: Lactoferrin is found in mucus, milk, and colostrum secretions and has antimicrobial activities, improves iron absorption, and enhances immune responses. Lactoferrin has the ability to degrade starch. The aim of this study was to investigate the preservation of milk lactoferrin enzymatic activity after purification by ion exchange chromatography. Materials and methods: ...
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The quantum yield of singlet oxygen production was investigated in dodecylammonium propionate reversed micellar solutions containing 1,3-diphenylisobenzofuran and fluorescein as photosensitizer. The quantum yield was highly enhanced by the binding of fluorescein to reversed micelles, and increased with decreasing the solubilized water content in reversed micelles. Results obtained was discussed...
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Affinity Cibacron Blue 3GA (CB) dye in aqueous phase was directly transferred to the reversed micelles due to electrostatic interaction between anionic CB and cationic cetyltrimethylammonium bromide (CTAB). The bovine serum albumin (BSA) transfer to the reverse micelles increases significantly in a wide range of pH by the addition of a small amount of CB ( approximately 1.0-7.0% of the total su...
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ژورنال
عنوان ژورنال: Biotechnology and Bioengineering
سال: 1995
ISSN: 0006-3592,1097-0290
DOI: 10.1002/bit.260480624